NSJ Bioreagents
SKU:V9696-100UG
Actin alpha 1 Antibody / ACTA1, 100 ug
Actin alpha 1 Antibody / ACTA1, 100 ug
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All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Specifications
| Catalog No | V9696-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | ACTA1/360 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Species Reactivity | Human (broad species reactivity predicted) |
| Application | IHC-P, WB |
| Application Details | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml |
| Application Note | Optimal dilution of the Actin alpha 1 antibody should be determined by the researcher. |
| Localization | Cytoplasm |
| Immunogen | Recombinant human ACTA1 protein was used as the immunogen for the Actin alpha 1 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Actin alpha 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Actin alpha 1 antibody is available for research use only. |
| Uniprot # | P68133 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/actin-alpha-1-antibody-acta1-acta1360-v9696 |
| Title | Actin alpha 1 Antibody / ACTA1 |
| Description | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. |
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