NSJ Bioreagents
SKU:V9690-100UG
Neurogenin 3 Antibody / NGN3, 100 ug
Neurogenin 3 Antibody / NGN3, 100 ug
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This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.
Specifications
| Catalog No | V9690-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-NEUROG3-1E10 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Species Reactivity | Human |
| Application | ELISA, IF, FACS |
| Application Details | ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells |
| Application Note | Optimal dilution of the Neurogenin 3 antibody should be determined by the researcher. |
| Localization | Nucleus |
| Immunogen | Recombinant human full-length protein was used as the immunogen for the Neurogenin 3 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Neurogenin 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Neurogenin 3 antibody is available for research use only. |
| Uniprot # | Q9Y4Z2 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/neurogenin-3-antibody-ngn3-pcrp-neurog3-1e10-v9690 |
| Title | Neurogenin 3 Antibody / NGN3 |
| Description | This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter. |
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