NSJ Bioreagents
SKU:V9686-100UG
NFIA Antibody / Nuclear Factor 1 A, 100 ug
NFIA Antibody / Nuclear Factor 1 A, 100 ug
Couldn't load pickup availability
Nuclear factor (NF) proteins are a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, -globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
Specifications
| Catalog No | V9686-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-NFIA-2C6 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2c |
| Species Reactivity | Human |
| Application | FACS, IF, WB, IHC-P |
| Application Details | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the NFIA antibody should be determined by the researcher. |
| Localization | Nucleus |
| Immunogen | A recombinant protein fragment was used as the immunogen for the NFIA antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the NFIA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This NFIA antibody is available for research use only. |
| Uniprot # | Q12857 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/nfia-antibody-nuclear-factor-1-a-pcrp-nfia-2c6-v9686 |
| Title | NFIA Antibody / Nuclear Factor 1 A |
| Description | Nuclear factor (NF) proteins are a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, -globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter. |
Share
