NSJ Bioreagents
SKU:V9684-20UG
Myf-4 Antibody / MYOG / Myogenin, 20 ug
Myf-4 Antibody / MYOG / Myogenin, 20 ug
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Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
Specifications
| Catalog No | V9684-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-MYOG-1C5 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Species Reactivity | Human |
| Application | ELISA, FACS, IF, WB |
| Application Details | ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml |
| Application Note | Optimal dilution of the Myf-4 antibody should be determined by the researcher. |
| Localization | Nucleus |
| Immunogen | Human recombinant protein was used as the immunogen for the Myf-4 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Myf-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Myf-4 antibody is available for research use only. |
| Uniprot # | P15173 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/myf-4-antibody-myog-myogenin-pcrp-myog-1c5-v9684 |
| Title | Myf-4 Antibody / MYOG / Myogenin |
| Description | Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor. |
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