NSJ Bioreagents
SKU:V9666-100UG
Keratin 14 Antibody / Cytokeratin 14 / KRT14, 100 ug
Keratin 14 Antibody / Cytokeratin 14 / KRT14, 100 ug
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Cytokeratin 14 is a member of the type I keratin family of intermediate filament proteins. It always pairs with the type II keratin K5 and form the primary keratin pair of the keratinocytes of stratified squamous epithelia, including the epidermis as well as mucosal non-keratinizing stratified squamous epithelia.Cytokeratin 14 is strongly expressed in the undifferentiated basal cell layer containing the stem cells and are down-regulated in the differentiating suprabasal cell layers. Otherwise, in the widely well stratified follicular outer root sheath, cytokeratin 14 is uniformly expressed throughout all layers. The expression spectrum of cytokeratin 14 in tumors corresponds well to the patterns in normal epithelia. Thus, most squamous cell carcinomas as well as malignant mesotheliomas strongly express this keratin whereas little, focal, or no expression is found in adenocarcinomas.Cytokeratin 14 may be a useful marker in the differential diagnosis of squamous cell carcinoma from other epithelial tumors. Recent studies also indicate that CK14 expression in breast cancer corresponded with poor clinical outcome and that CK14 may have diagnostic value in the sub-classification of NSCLC.
Specifications
| Catalog No | V9666-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | KRT14/4584R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Application | IHC-P |
| Application Details | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the Keratin 14 antibody should be determined by the researcher. |
| Localization | Cytoplasm |
| Immunogen | A portion of amino acids 350-472 was used as the immunogen for the Keratin 14 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Keratin 14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Keratin 14 antibody is available for research use only. |
| Uniprot # | P02533 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/keratin-14-antibody-cytokeratin-14-krt14-krt144584r-v9666 |
| Title | Keratin 14 Antibody / Cytokeratin 14 / KRT14 |
| Description | Cytokeratin 14 is a member of the type I keratin family of intermediate filament proteins. It always pairs with the type II keratin K5 and form the primary keratin pair of the keratinocytes of stratified squamous epithelia, including the epidermis as well as mucosal non-keratinizing stratified squamous epithelia.Cytokeratin 14 is strongly expressed in the undifferentiated basal cell layer containing the stem cells and are down-regulated in the differentiating suprabasal cell layers. Otherwise, in the widely well stratified follicular outer root sheath, cytokeratin 14 is uniformly expressed throughout all layers. The expression spectrum of cytokeratin 14 in tumors corresponds well to the patterns in normal epithelia. Thus, most squamous cell carcinomas as well as malignant mesotheliomas strongly express this keratin whereas little, focal, or no expression is found in adenocarcinomas.Cytokeratin 14 may be a useful marker in the differential diagnosis of squamous cell carcinoma from other epithelial tumors. Recent studies also indicate that CK14 expression in breast cancer corresponded with poor clinical outcome and that CK14 may have diagnostic value in the sub-classification of NSCLC. |
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