• IHC staining of FFPE human thyroid tissue with HOXB2 antibody (clone PCRP-HOXB2-1F2) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9653-100UG

HOXB2 Antibody, 100 ug

HOXB2 Antibody, 100 ug

$559.00 USD
$559.00 USD
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Hox genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene HoxB1 is critical to hindbrain development and has phenotypic features frequently observed in autism. Analysis of expression and targeted disruption of HoxB1 demonstrates that it is also essential for patterning progenitor cells along the entire DV axis of rhombomere 4 (r4). HoxB1 maintains this function by acting very early during hindbrain neurogenesis to specify effectors of the Sonic hedgehog and Mash1 signaling pathways. HoxB2 is a homeodomain protein important in neural development that is also expressed during erythropoiesis, hindbrain development and normal human adult lung development. HoxB2 may modulate the amount of gamma-globin mRNA expressed during development and differentiation. In addition, HoxB2 plays an important role in the patterning of hindbrain and pharyngeal arches in the zebrafish.

Specifications

Catalog No V9653-100UG
Family Primary antibody
Qty 100 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone PCRP-HOXB2-1F2
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Species Reactivity Human
Application ELISA, FACS, IF, WB, IHC-P
Application Details ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the HOXB2 antibody should be determined by the researcher.
Immunogen A portion of amino acids 114-220 was used as the immunogen for the HOXB2 antibody.
Purity Protein A/G affinity
Storage Aliquot the HOXB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This HOXB2 antibody is available for research use only.
Uniprot # P14652
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/hoxb2-antibody-pcrp-hoxb2-1f2-v9653
Title HOXB2 Antibody
Description Hox genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene HoxB1 is critical to hindbrain development and has phenotypic features frequently observed in autism. Analysis of expression and targeted disruption of HoxB1 demonstrates that it is also essential for patterning progenitor cells along the entire DV axis of rhombomere 4 (r4). HoxB1 maintains this function by acting very early during hindbrain neurogenesis to specify effectors of the Sonic hedgehog and Mash1 signaling pathways. HoxB2 is a homeodomain protein important in neural development that is also expressed during erythropoiesis, hindbrain development and normal human adult lung development. HoxB2 may modulate the amount of gamma-globin mRNA expressed during development and differentiation. In addition, HoxB2 plays an important role in the patterning of hindbrain and pharyngeal arches in the zebrafish.
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