NSJ Bioreagents
SKU:V9649-20UG
GTF2A1 Antibody / TFIIA, 20 ug
GTF2A1 Antibody / TFIIA, 20 ug
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Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.
Specifications
| Catalog No | V9649-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-GTF2A1-1F2 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Species Reactivity | Human |
| Application | ELISA, FACS, IF |
| Application Details | ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml |
| Application Note | Optimal dilution of the GTF2A1 antibody should be determined by the researcher. |
| Localization | Nucleus, cytoplasm |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the GTF2A1 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the GTF2A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This GTF2A1 antibody is available for research use only. |
| Uniprot # | P52655 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/gtf2a1-antibody-tfiia-pcrp-gtf2a1-1f2-v9649 |
| Title | GTF2A1 Antibody / TFIIA |
| Description | Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene. |
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