NSJ Bioreagents
SKU:V9621-20UG
Decapping Protein 2 Antibody / DCP2 / m7GpppN-mRNA hydrolase, 20 ug
Decapping Protein 2 Antibody / DCP2 / m7GpppN-mRNA hydrolase, 20 ug
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The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay
Specifications
| Catalog No | V9621-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-DCP2-1D6 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a |
| Species Reactivity | Human |
| Application | FACS, IF |
| Application Details | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml |
| Application Note | Optimal dilution of the Decapping Protein 2 antibody should be determined by the researcher. |
| Localization | Nucleus, Cytoplasm |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the Decapping Protein 2 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Decapping Protein 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Decapping Protein 2 antibody is available for research use only. |
| Uniprot # | Q8IU60 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/decapping-protein-2-antibody-dcp2-m7gpppn-mrna-hydrolase-pcrp-dcp2-1d6-v9621 |
| Title | Decapping Protein 2 Antibody / DCP2 / m7GpppN-mRNA hydrolase |
| Description | The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay |
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