• IHC staining of FFPE human placental tissue with Transglutaminase 2 antibody (clone TGM2/3612). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9619-20UG

Transglutaminase 2 Antibody (FN Binding Domain), 20 ug

Transglutaminase 2 Antibody (FN Binding Domain), 20 ug

$259.00 USD
$259.00 USD
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Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues. Extracellular tTG is able to bind to and cross-link several ECM proteins including FN/Fibronectin and SPP1, and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation. High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.

Specifications

Catalog No V9619-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone TGM2/3612
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Species Reactivity Human
Application FACS, IF, IHC-P
Application Details Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the Transglutaminase 2 antibody should be determined by the researcher.
Localization Cytoplasm, Cell surface
Immunogen Recombinant full-length human TGM2 protein was used as the immunogen for the Transglutaminase 2 antibody.
Purity Protein A/G affinity
Storage Aliquot the Transglutaminase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Transglutaminase 2 antibody is available for research use only.
Uniprot # P21980
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/transglutaminase-2-antibody-fn-binding-domain-tgm23612-v9619
Title Transglutaminase 2 Antibody (FN Binding Domain)
Description Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues. Extracellular tTG is able to bind to and cross-link several ECM proteins including FN/Fibronectin and SPP1, and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation. High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.
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