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NSJ Bioreagents
SKU:V9615-100UG
Recombinant Vinculin Antibody, 100 ug
Recombinant Vinculin Antibody, 100 ug
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Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Specifications
| Catalog No | V9615-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | VCL/7091R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse |
| Application | WB, IHC-P |
| Application Details | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the recombinant Vinculin antibody should be determined by the researcher. |
| Localization | Cytoplasm |
| Immunogen | A portion of amino acids 174-322 was used as the immunogen for the recombinant Vinculin antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the recombinant Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This recombinant Vinculin antibody is available for research use only. |
| Uniprot # | P18206 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/recombinant-vinculin-antibody-vcl7091r-v9615 |
| Title | Recombinant Vinculin Antibody |
| Description | Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin. |
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