• IHC staining of FFPE human renal carcinoma tissue with SREBF2 antibody (clone SREBP2/1580) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9612-20UG

SREBF2 Antibody / SREBP2, 20 ug

SREBF2 Antibody / SREBP2, 20 ug

$259.00 USD
$259.00 USD
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The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5 flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix-leucine zipper (bHLH-zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way.

Specifications

Catalog No V9612-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone SREBP2/1580
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the SREBF2 antibody should be determined by the researcher.
Localization Nucleus, Cytoplasm
Immunogen Recombinant full-length human protein was used as the immunogen for the SREBF2 antibody.
Purity Protein A/G affinity
Storage Aliquot the SREBF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This SREBF2 antibody is available for research use only.
Uniprot # Q12772
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/srebf2-antibody-srebp2-srebp21580-v9612
Title SREBF2 Antibody / SREBP2
Description The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5 flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix-leucine zipper (bHLH-zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way.
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