NSJ Bioreagents
SKU:V9597-20UG
Tyrosinase Antibody, 20 ug
Tyrosinase Antibody, 20 ug
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Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants.This MAb is a useful marker for melanocytes and melanomas.
Specifications
| Catalog No | V9597-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | TYR/3826 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Species Reactivity | Human |
| Application | IHC-P |
| Application Details | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the Tyrosinase antibody should be determined by the researcher. |
| Localization | Cytoplasm |
| Immunogen | Recombinant full-length human Tyrosinase protein was used as the immunogen for the Tyrosinase antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Tyrosinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Tyrosinase antibody is available for research use only. |
| Uniprot # | P14679 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/tyrosinase-antibody-tyr3826-v9597 |
| Title | Tyrosinase Antibody |
| Description | Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants.This MAb is a useful marker for melanocytes and melanomas. |
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