NSJ Bioreagents
SKU:V9589-20UG
SPARC Antibody / Osteonectin, 20 ug
SPARC Antibody / Osteonectin, 20 ug
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SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Specifications
| Catalog No | V9589-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | OSTN/3759 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2c, kappa |
| Species Reactivity | Human |
| Application | WB, IHC-P |
| Application Details | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the SPARC antibody should be determined by the researcher. |
| Localization | Secreted |
| Immunogen | A portion of amino acids 1-200 was used as the immunogen for the SPARC antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This SPARC antibody is available for research use only. |
| Uniprot # | P09486 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/sparc-antibody-osteonectin-ostn3759-v9589 |
| Title | SPARC Antibody / Osteonectin |
| Description | SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules. |
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