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NSJ Bioreagents
SKU:V9569-20UG
RPA32 Antibody / RPA2 / Replication Protein A2, 20 ug
RPA32 Antibody / RPA2 / Replication Protein A2, 20 ug
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Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Specifications
| Catalog No | V9569-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | RPA2/4774 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Species Reactivity | Human |
| Application | WB, IHC-P |
| Application Details | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the RPA32 antibody should be determined by the researcher. |
| Localization | Nuclear |
| Immunogen | Recombinant human full-length Replication Protein A2 protein was used as the immunogen for the RPA32 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the RPA32 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This RPA32 antibody is available for research use only. |
| Uniprot # | P15927 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/rpa32-antibody-rpa2-replication-protein-a2-rpa24774-v9569 |
| Title | RPA32 Antibody / RPA2 / Replication Protein A2 |
| Description | Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
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