NSJ Bioreagents
SKU:V9540-20UG
Recombinant PSA Antibody / Prostate Specific Antigen, 20 ug
Recombinant PSA Antibody / Prostate Specific Antigen, 20 ug
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Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8% carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells. Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99% of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1% of poorly differentiated carcinomas have been negative for PSA. Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.
Specifications
| Catalog No | V9540-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | KLK3/4602R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Application | IHC-P |
| Application Details | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the recombinant PSA antibody should be determined by the researcher. |
| Localization | Secreted |
| Immunogen | A portion of amino acids 150-250 of Prostate-specific antigen protein was used as the immunogen for the recombinant PSA antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the recombinant PSA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This recombinant PSA antibody is available for research use only. |
| Uniprot # | P07288 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/recombinant-psa-antibody-prostate-specific-antigen-klk34602r-v9540 |
| Title | Recombinant PSA Antibody / Prostate Specific Antigen |
| Description | Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8% carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells. Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99% of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1% of poorly differentiated carcinomas have been negative for PSA. Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA. |
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