NSJ Bioreagents
SKU:V9490-100UG
DNTT Antibody / TdT / DNA Nucleotidylexotransferase, 100 ug
DNTT Antibody / TdT / DNA Nucleotidylexotransferase, 100 ug
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Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).
Specifications
| Catalog No | V9490-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | rDNTT/6909 |
| Host Animal | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Species Reactivity | Human |
| Application | ELISA, IHC-P |
| Application Details | ELISA (Use BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the DNTT antibody should be determined by the researcher. |
| Localization | Nucleus |
| Immunogen | A portion of amino acids 1-100 was used as the immunogen for the DNTT antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the DNTT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This DNTT antibody is available for research use only. |
| Uniprot # | P04053 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/dntt-antibody-tdt-dna-nucleotidylexotransferase-rdntt6909-v9490 |
| Title | DNTT Antibody / TdT / DNA Nucleotidylexotransferase |
| Description | Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL). |
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