• IHC staining of FFPE human colon tissue with p27Kip1 antibody (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9478-20UG

p27Kip1 Antibody / p27 / CDKN1B, 20 ug

p27Kip1 Antibody / p27 / CDKN1B, 20 ug

$259.00 USD
$259.00 USD
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This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Specifications

Catalog No V9478-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone KIP1/1357
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the p27Kip1 antibody should be determined by the researcher.
Localization Nuclear
Immunogen Recombinant full-length human CDKN1B protein was used as the immunogen for the p27Kip1 antibody.
Purity Protein A/G affinity
Storage Aliquot the p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This p27Kip1 antibody is available for research use only.
Uniprot # P46527
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/p27kip1-antibody-p27-cdkn1b-kip11357-v9478
Title p27Kip1 Antibody / p27 / CDKN1B
Description This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
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