• IHC staining of FFPE human placental tissue with Fibroblast Activation Protein Alpha antibody (clone FAP/4851). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9470-20UG

Fibroblast Activation Protein Alpha Antibody / FAP, 20 ug

Fibroblast Activation Protein Alpha Antibody / FAP, 20 ug

$259.00 USD
$259.00 USD
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FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is colocalized with MMP-1 and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.

Specifications

Catalog No V9470-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone FAP/4851
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the Fibroblast Activation Protein Alpha antibody should be determined by the researcher.
Localization Cell surface, Cell membrane
Immunogen A portion of amino acids 1-200 was used as the immunogen for the Fibroblast Activation Protein Alpha antibody.
Purity Protein A/G affinity
Storage Aliquot the Fibroblast Activation Protein Alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Fibroblast Activation Protein Alpha antibody is available for research use only.
Uniprot # Q12884
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/fibroblast-activation-protein-alpha-antibody-fap-fap4851-v9470
Title Fibroblast Activation Protein Alpha Antibody / FAP
Description FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is colocalized with MMP-1 and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.
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