• IHC staining of FFPE human placental tissue with ALPP antibody (clone ALPP/4109) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9467-100UG

ALPP Antibody / Placental Alkaline Phosphatase, 100 ug

ALPP Antibody / Placental Alkaline Phosphatase, 100 ug

$559.00 USD
$559.00 USD
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Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Specifications

Catalog No V9467-100UG
Family Primary antibody
Qty 100 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone ALPP/4109
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the ALPP antibody should be determined by the researcher.
Localization Cytoplasmic, Cell surface
Immunogen Recombinant full-length human ALPP protein was used as the immunogen for the ALPP antibody.
Purity Protein A/G affinity
Storage Aliquot the ALPP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This ALPP antibody is available for research use only.
Uniprot # P05187
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/alpp-antibody-placental-alkaline-phosphatase-alpp4109-v9467
Title ALPP Antibody / Placental Alkaline Phosphatase
Description Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
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