NSJ Bioreagents
SKU:V9459-100UG
Recombinant CD3e Antibody / CD3 epsilon, 100 ug
Recombinant CD3e Antibody / CD3 epsilon, 100 ug
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CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Specifications
| Catalog No | V9459-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | C3e/4652R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Application | ELISA, WB, IHC-P |
| Application Details | ELISA: 2-4ug/ml (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the recombinant CD3e antibody should be determined by the researcher. |
| Localization | Cell surface, Cytoplasm |
| Immunogen | A portion of amino acids aa 20-40 from the human protein was used as the immunogen for the recombinant CD3e antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the recombinant CD3e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This recombinant CD3e antibody is available for research use only. |
| Uniprot # | P07766 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/recombinant-cd3e-antibody-cd3-epsilon-c3e4652r-v9459 |
| Title | Recombinant CD3e Antibody / CD3 epsilon |
| Description | CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. |
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