• IHC staining of FFPE human skeletal muscle with recombinant Sarcomeric Alpha Actinin antibody (clone ACTN2/7041R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9433-20UG

Recombinant Sarcomeric Alpha Actinin Antibody / Alpha Actinin 2 / ACTN2, 20 ug

Recombinant Sarcomeric Alpha Actinin Antibody / Alpha Actinin 2 / ACTN2, 20 ug

$259.00 USD
$259.00 USD
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Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Specifications

Catalog No V9433-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone ACTN2/7041R
Host Animal Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant Sarcomeric Alpha Actinin antibody should be determined by the researcher.
Localization Cytoplasm
Immunogen A portion of amino acids 557-692 from the human protein was used as the immunogen for the recombinant Sarcomeric Alpha Actinin antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant Sarcomeric Alpha Actinin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant Sarcomeric Alpha Actinin antibody is available for research use only.
Uniprot # P35609
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-sarcomeric-alpha-actinin-antibody-alpha-actinin-2-actn2-actn27041r-v9433
Title Recombinant Sarcomeric Alpha Actinin Antibody / Alpha Actinin 2 / ACTN2
Description Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
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