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NSJ Bioreagents
SKU:V9420-100UG
Recombinant Actin Antibody / ACTA2 / Smooth Muscle, 100 ug
Recombinant Actin Antibody / ACTA2 / Smooth Muscle, 100 ug
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Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Specifications
| Catalog No | V9420-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | ACTA2/1614R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Species Reactivity | Human |
| Application | IHC-P, WB |
| Application Details | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml |
| Application Note | Optimal dilution of the recombinant Actin antibody should be determined by the researcher. |
| Localization | Cytoplasmic |
| Immunogen | Recombinant full-length human ACTA2 protein was used as the immunogen for the recombinant Actin antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the recombinant Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This recombinant Actin antibody is available for research use only. |
| Uniprot # | P62736 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/recombinant-actin-antibody-acta2-smooth-muscle-acta21614r-v9420 |
| Title | Recombinant Actin Antibody / ACTA2 / Smooth Muscle |
| Description | Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin. |
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