NSJ Bioreagents
SKU:V9418-100UG
CHRAC17 Antibody / POLE3, 100 ug
CHRAC17 Antibody / POLE3, 100 ug
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DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.
Specifications
| Catalog No | V9418-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-POLE3-3D3 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a |
| Species Reactivity | Human |
| Application | ELISA, FACS, IF |
| Application Details | ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml |
| Application Note | Optimal dilution of the CHRAC17 antibody should be determined by the researcher. |
| Localization | Nucleus, Cytoplasm |
| Immunogen | Recombinant full-length human POLE3 protein was used as the immunogen for the CHRAC17 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the CHRAC17 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This CHRAC17 antibody is available for research use only. |
| Uniprot # | Q9NRF9 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/chrac17-antibody-pole3-pcrp-pole3-3d3-v9418 |
| Title | CHRAC17 Antibody / POLE3 |
| Description | DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure. |
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