• IHC staining of FFPE human cerebellum tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/4450). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9403-20UG

Glial Fibrillary Acidic Protein Antibody / GFAP, 20 ug

Glial Fibrillary Acidic Protein Antibody / GFAP, 20 ug

$259.00 USD
$259.00 USD
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GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Specifications

Catalog No V9403-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone GFAP/4450
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Species Reactivity Human
Application IHC-P, WB
Application Details Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Application Note Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.
Localization Cytoplasm
Immunogen Recombinant full-length human GFAP protein was used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
Purity Protein A/G affinity
Storage Aliquot the Glial Fibrillary Acidic Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Glial Fibrillary Acidic Protein antibody is available for research use only.
Uniprot # P14136
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/glial-fibrillary-acidic-protein-antibody-gfap-gfap4450-v9403
Title Glial Fibrillary Acidic Protein Antibody / GFAP
Description GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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