• IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9392-20UG

Recombinant ERa Antibody / Estrogen Receptor alpha, 20 ug

Recombinant ERa Antibody / Estrogen Receptor alpha, 20 ug

$259.00 USD
$259.00 USD
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The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.

Specifications

Catalog No V9392-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone ESR1/4039R
Host Animal Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant ERa antibody should be determined by the researcher.
Localization Nucleus
Immunogen A recombinant protein fragment from the C-terminal region was used as the immunogen for the recombinant ERa antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant ERa antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant ERa antibody is available for research use only.
Uniprot # P03372
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-era-antibody-estrogen-receptor-alpha-esr14039r-v9392
Title Recombinant ERa Antibody / Estrogen Receptor alpha
Description The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.
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