NSJ Bioreagents
SKU:V9391-100UG
AGO3 Antibody / Argonaute-3 / eIF2C3, 100 ug
AGO3 Antibody / Argonaute-3 / eIF2C3, 100 ug
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This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as Argonaute RISC catalytic component 3. Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5-flanking sequences to the region of guide-target complementarity.
Specifications
| Catalog No | V9391-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-AGO3-1C5 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Species Reactivity | Human |
| Application | ELISA, FACS, IF |
| Application Details | ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml |
| Application Note | Optimal dilution of the AGO3 antibody should be determined by the researcher. |
| Localization | Cytoplasm, Nucleus |
| Immunogen | Recombinant full-length human AGO3 protein was used as the immunogen for the AGO3 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the AGO3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This AGO3 antibody is available for research use only. |
| Uniprot # | Q9H9G7 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/ago3-antibody-argonaute-3-eif2c3-pcrp-ago3-1c5-v9391 |
| Title | AGO3 Antibody / Argonaute-3 / eIF2C3 |
| Description | This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as Argonaute RISC catalytic component 3. Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5-flanking sequences to the region of guide-target complementarity. |
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