• IHC staining of FFPE human testis with recombinant Oct4 antibody (clone OCT4/6875R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9377-20UG

Recombinant Oct4 Antibody / POU5F1, 20 ug

Recombinant Oct4 Antibody / POU5F1, 20 ug

$259.00 USD
$259.00 USD
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OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Specifications

Catalog No V9377-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone OCT4/6875R
Host Animal Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant antibody should be determined by the researcher.
Localization Nucleus
Immunogen A portion of amino acids1-134 was used as the immunogen for the recombinant Oct4 antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant Oct4 antibody is available for research use only.
Uniprot # Q01860
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-oct4-antibody-pou5f1-oct46875r-v9377
Title Recombinant Oct4 Antibody / POU5F1
Description OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.
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