NSJ Bioreagents
SKU:V9371-100UG
Oct4 Antibody / POU5F1, 100 ug
Oct4 Antibody / POU5F1, 100 ug
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Oct4 Transcription factor that binds to the octamer motif (5). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Specifications
| Catalog No | V9371-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | Oct4/3508 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Species Reactivity | Human |
| Application | IHC-P |
| Application Details | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the Oct4 antibody should be determined by the researcher. |
| Localization | Nucleus |
| Immunogen | A portion of amino acids 1-134 was used as the immunogen for the Oct4. antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Oct4 antibody is available for research use only. |
| Uniprot # | Q01860 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/oct4-antibody-pou5f1-oct43508-v9371 |
| Title | Oct4 Antibody / POU5F1 |
| Description | Oct4 Transcription factor that binds to the octamer motif (5). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
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