NSJ Bioreagents
SKU:V9362-20UG
Recombinant Pan-CK Antibody Cocktail, 20 ug
Recombinant Pan-CK Antibody Cocktail, 20 ug
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Anti-cytokeratin clone rPCK/6750 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
Specifications
| Catalog No | V9362-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | Cocktail rPCK/6750 |
| Host Animal | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Species Reactivity | Human |
| Application | FACS, IF, IHC-P |
| Application Details | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the recombinant Pan-CK antibody should be determined by the researcher. |
| Localization | Cytoplasmic |
| Immunogen | Crude cytokeratin extract prepared from RT-4 and MCF-7 cells was used as the immunogen for the recombinant Pan-CK antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the recombinant Pan-CK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This recombinant Pan-CK antibody is available for research use only. |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/recombinant-pan-ck-antibody-cocktail-cocktail-rpck6750-v9362 |
| Title | Recombinant Pan-CK Antibody Cocktail |
| Description | Anti-cytokeratin clone rPCK/6750 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells. |
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