• IHC staining of FFPE human prostate tissue with recombinant Pan Cytokeratin antibody (clone PCK/4933R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9361-20UG

Recombinant Pan Cytokeratin Antibody Cocktail, 20 ug

Recombinant Pan Cytokeratin Antibody Cocktail, 20 ug

$259.00 USD
$259.00 USD
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PCK/4933R demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/4933R is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/4933R is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Specifications

Catalog No V9361-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone Cocktail PCK/4933R
Host Animal Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant Pan Cytokeratin antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen Crude cytokeratin extract prepared from RT-4 and MCF-7 cells was used as the immunogen for the recombinant Pan Cytokeratin antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant Pan Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant Pan Cytokeratin antibody is available for research use only.
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-pan-cytokeratin-antibody-cocktail-cocktail-pck4933r-v9361
Title Recombinant Pan Cytokeratin Antibody Cocktail
Description PCK/4933R demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/4933R is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/4933R is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
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