• IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN/36). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9352-100UG

Biotin Antibody, 100 ug

Biotin Antibody, 100 ug

$559.00 USD
$559.00 USD
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It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Specifications

Catalog No V9352-100UG
Family Primary antibody
Qty 100 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone BTN/36
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Species Reactivity All species
Application FACS, IF, WB, IHC-P
Application Details Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note Optimal dilution of the Biotin antibody should be determined by the researcher.
Immunogen Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Purity Protein A/G affinity
Storage Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Biotin antibody is available for research use only.
Uniprot # Not Applicable
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/biotin-antibody-btn36-v9352
Title Biotin Antibody
Description It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
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