• IHC staining of FFPE human skin with recombinant Basic Cytokeratin antibody (clone rKRTH/6617). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9305-20UG

Recombinant Basic Cytokeratin Antibody (HMW / Type II), 20 ug

Recombinant Basic Cytokeratin Antibody (HMW / Type II), 20 ug

$259.00 USD
$259.00 USD
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The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Specifications

Catalog No V9305-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone rKRTH/6617
Host Animal Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen A synthetic peptide sequence common to Type II Cytokeratins was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant Basic Cytokeratin antibody is available for research use only.
Uniprot # Q01546
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-basic-cytokeratin-antibody-hmw-type-ii-rkrth6617-v9305
Title Recombinant Basic Cytokeratin Antibody (HMW / Type II)
Description The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
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