• IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9300-100UG

Recombinant Acidic Cytokeratin Antibody (LMW / Type I), 100 ug

Recombinant Acidic Cytokeratin Antibody (LMW / Type I), 100 ug

$559.00 USD
$559.00 USD
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The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. Its usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Specifications

Catalog No V9300-100UG
Family Primary antibody
Qty 100 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone rKRTL/6616
Host Animal Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant Acidic Cytokeratin antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen A synthetic peptide sequence common to Type I Cytokeratins was used as the immunogen for the recombinant Acidic Cytokeratin antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant Acidic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant Acidic Cytokeratin antibody is available for research use only.
Uniprot # Q7Z794
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-acidic-cytokeratin-antibody-lmw-type-i-rkrtl6616-v9300
Title Recombinant Acidic Cytokeratin Antibody (LMW / Type I)
Description The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. Its usually used in a cytokeratin cocktail with type II cytokeratin antibody.
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