• IHC staining of FFPE human lymph node with CD48 antibody (clone CD48/4784). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9298-20UG

CD48 Antibody (Pan Leukocyte Marker), 20 ug

CD48 Antibody (Pan Leukocyte Marker), 20 ug

$259.00 USD
$259.00 USD
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Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Specifications

Catalog No V9298-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone CD48/4784
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c, kappa
Species Reactivity Human
Application WB, IHC-P
Application Details Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the CD48 antibody should be determined by the researcher.
Localization Cell Surface
Immunogen A portion of amino acids 59-178 was used as the immunogen for the CD48 antibody.
Purity Protein A/G affinity
Storage Aliquot the CD48 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This CD48 antibody is available for research use only.
Uniprot # P09326
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cd48-antibody-pan-leukocyte-marker-cd484784-v9298
Title CD48 Antibody (Pan Leukocyte Marker)
Description Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.
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