NSJ Bioreagents
SKU:V9293-100UG
Vimentin Antibody, 100 ug
Vimentin Antibody, 100 ug
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Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Specifications
| Catalog No | V9293-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | rVIM/6575 |
| Host Animal | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Species Reactivity | Human (broad species reactivity predicted) |
| Application | WB, IHC-P |
| Application Details | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the Vimentin antibody should be determined by the researcher. |
| Localization | Cytoplasmic |
| Immunogen | Recombinant full-length human Vimentin protein was used as the immunogen for the Vimentin antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Vimentin antibody is available for research use only. |
| Uniprot # | P08670 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/vimentin-antibody-rvim6575-v9293 |
| Title | Vimentin Antibody |
| Description | Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. |
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