• IHC staining of FFPE human lymph node with LEF1 antibody (clone LEF1/341R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9278-20UG

LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha, 20 ug

LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha, 20 ug

$259.00 USD
$259.00 USD
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Lymphoid Enhancing Factor 1 (LEF1), also called T cell-specific transcription factor 1-alpha (TCF1 alpha) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.

Specifications

Catalog No V9278-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone LEF1/341R
Host Animal Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 2-4ug/ml
Application Note Optimal dilution of the LEF1 antibody should be determined by the researcher.
Localization Nucleus
Immunogen A portion of amino acids 100-200 was used as the immunogen for the LEF1 antibody.
Purity Protein A/G affinity
Storage Aliquot the LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This LEF1 antibody is available for research use only.
Uniprot # Q9UJU2
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/lef1-antibody-lymphoid-enhancer-binding-factor-1-tcf1-alpha-lef1341r-v9278
Title LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha
Description Lymphoid Enhancing Factor 1 (LEF1), also called T cell-specific transcription factor 1-alpha (TCF1 alpha) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.
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