• IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone CD47/6362R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9270-20UG

Recombinant CD47 Antibody / IAP / Integrin Associated Protein, 20 ug

Recombinant CD47 Antibody / IAP / Integrin Associated Protein, 20 ug

$259.00 USD
$259.00 USD
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This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Specifications

Catalog No V9270-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone CD47/6362R
Host Animal Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Species Reactivity Human
Application ELISA, IHC-P
Application Details ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the recombinant CD47 antibody should be determined by the researcher.
Localization Cell surface, cytoplasmic
Immunogen A portion of amino acids 18-135 from the human protein was used as the immunogen for the recombinant CD47 antibody.
Purity Protein A/G affinity
Storage Aliquot the recombinant CD47 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This recombinant CD47 antibody is available for research use only.
Uniprot # Q08722
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/recombinant-cd47-antibody-iap-integrin-associated-protein-cd476362r-v9270
Title Recombinant CD47 Antibody / IAP / Integrin Associated Protein
Description This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
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