PTGER3 Antibody / Prostaglandin E2 receptor EP3, 100 ug

PTGER3 antibody detects Prostaglandin E2 receptor EP3 subtype, a G protein-coupled receptor (GPCR) encoded by the PTGER3 gene located on chromosome 1p31.1. PTGER3 mediates the biological effects of prostaglandin E2 (PGE2), a key lipid signaling molecule involved in inflammation, pain perception, smooth muscle contraction, and thermoregulation. The EP3 receptor is unique among the four PGE2 receptor subtypes (EP1-EP4) because of its multiple isoforms generated through alternative splicing, each coupling to different G proteins (Gi, Gs, or Gq) and eliciting diverse downstream responses.

Structurally, PTGER3 is a seven-transmembrane GPCR with extracellular ligand-binding domains and intracellular loops that interact with heterotrimeric G proteins. It belongs to the rhodopsin-like GPCR family, sharing sequence homology with other prostanoid receptors. Co-localization studies show PTGER3 localized on the plasma membrane of smooth muscle cells, immune cells, and neurons. The receptor�s cytoplasmic tail contains regions that determine isoform-specific signaling and regulatory properties.

Functionally, PTGER3 mediates PGE2-dependent inhibition of adenylate cyclase via Gi coupling, leading to decreased intracellular cAMP levels. In smooth muscle, this signaling pathway promotes contraction, contributing to uterine and gastrointestinal motility. In the central nervous system, PTGER3 influences fever generation and pain modulation through hypothalamic pathways. The receptor also regulates renal sodium excretion, vascular tone, and platelet aggregation. Known ligands include PGE2 and selective agonists such as sulprostone and misoprostol.

PTGER3 expression is widespread but particularly high in uterus, kidney, stomach, and brain. Dysregulation of PTGER3 has been associated with inflammatory diseases, reproductive disorders, and cancer. Overexpression contributes to tumor progression by promoting angiogenesis and immune evasion, while inhibition reduces inflammation and hyperalgesia. Pathway associations include prostaglandin signaling, GPCR-mediated calcium signaling, and cAMP regulation. During development, PTGER3 contributes to uterine contractility and thermoregulatory responses.

The PTGER3 antibody from NSJ Bioreagents is a reliable reagent for studying prostaglandin signaling, GPCR pharmacology, and inflammatory regulation.