TRIM40 Antibody / Tripartite motif-containing protein 40, 100 ug

TRIM40 antibody detects Tripartite motif-containing protein 40, a member of the TRIM family of E3 ubiquitin ligases that regulate immune signaling, protein degradation, and cellular homeostasis. The TRIM40 gene encodes a cytosolic protein characterized by a RING finger domain, one or two B-box motifs, and a coiled-coil region, which together constitute the tripartite motif typical of this protein family. TRIM40 plays a crucial role in modulating NF-kappaB and interferon signaling pathways, acting as a negative regulator of innate immune responses.

Biochemical studies have demonstrated that TRIM40 mediates ubiquitination and degradation of components within the NF-kappaB pathway, including IKK gamma (NEMO), thereby dampening inflammatory signaling. It is expressed predominantly in gastrointestinal epithelial cells and immune tissues, where it contributes to immune tolerance and inflammation control. TRIM40 also regulates Rho GTPase signaling, maintaining cytoskeletal integrity and epithelial barrier function. Reduced expression or dysregulation of TRIM40 has been associated with inflammatory bowel disease, colorectal cancer, and autoimmune conditions, suggesting its role as a protective modulator of mucosal immunity.

Located on chromosome 6p21.33 within the MHC class I region, the TRIM40 gene is inducible by cytokines and microbial stimuli. The protein's E3 ligase activity enables it to tag target proteins for proteasomal degradation, ensuring tight control of inflammatory pathways. In addition to immune regulation, TRIM40 may influence oncogenic processes by modulating NF-kappaB-driven transcription of genes related to proliferation and survival. Overexpression studies indicate that TRIM40 stabilizes cytoskeletal structures and promotes cell adhesion, counteracting tumor cell invasiveness.

Immunostaining using TRIM40 antibody demonstrates cytoplasmic localization in intestinal epithelium and lymphoid tissues. The antibody serves as a valuable reagent for exploring immune signaling, epithelial cell regulation, and tumor suppression mechanisms. TRIM40 antibody from NSJ Bioreagents can be used in immunohistochemistry, western blotting, and other analytical applications to study the role of TRIM proteins in immune modulation and cancer biology.