GPX3 Antibody / Glutathione peroxidase 3, 100 ug

GPX3 antibody detects Glutathione peroxidase 3, a secreted antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides using glutathione. The UniProt recommended name is Glutathione peroxidase 3 (GPX3). This selenoprotein plays a key role in extracellular redox homeostasis and protection against oxidative damage in plasma and other biological fluids.

Functionally, GPX3 antibody identifies a 226-amino-acid glycoprotein containing a selenocysteine residue at its active site. GPX3 is primarily synthesized in the kidney proximal tubules and secreted into the plasma, where it scavenges peroxides to prevent oxidative injury to lipids, proteins, and DNA. Its catalytic activity depends on the presence of glutathione as a reducing cofactor and selenium availability for selenocysteine biosynthesis.

The GPX3 gene is located on chromosome 5q33.1 and encodes the only extracellular form among the glutathione peroxidase family. It is expressed in kidney, lung, liver, and several endocrine tissues, contributing to systemic antioxidant defense. GPX3 levels fluctuate with oxidative stress, inflammation, and selenium intake, making it a biomarker of redox status.

Pathologically, reduced GPX3 expression has been linked to cancer, diabetes, and cardiovascular diseases. Loss of GPX3 activity enhances oxidative DNA damage and supports tumor growth through redox imbalance. Conversely, elevated GPX3 expression in response to stress reflects a protective adaptation. Research with GPX3 antibody aids studies in oxidative stress, redox biology, and disease biomarker development.

GPX3 antibody is suitable for western blotting, immunohistochemistry, and ELISA to measure GPX3 protein in serum, plasma, and tissues. NSJ Bioreagents provides validated GPX3 antibody reagents optimized for oxidative stress, metabolism, and antioxidant pathway research.

Structurally, Glutathione peroxidase 3 forms a homotetramer with each subunit containing a conserved catalytic triad for peroxide reduction. The enzyme�s secretion signal sequence directs it to the extracellular space, where it complements intracellular antioxidant systems. This antibody enables quantitative and qualitative analysis of GPX3 in health and disease contexts.