NUSAP1 Antibody / Nucleolar and spindle-associated protein 1, 100 ug

NUSAP1 antibody detects Nucleolar and spindle-associated protein 1, a microtubule-binding protein essential for spindle formation and chromosome segregation during mitosis. The UniProt recommended name is Nucleolar and spindle-associated protein 1 (NUSAP1). This mitotic regulator links chromatin to microtubules, ensuring proper spindle assembly and stabilization required for accurate chromosome alignment and segregation.

Functionally, NUSAP1 antibody identifies a 495-amino-acid nuclear protein that localizes to the mitotic spindle and spindle poles. NUSAP1 binds microtubules through its C-terminal domain and bundles them into stable arrays, promoting bipolar spindle formation. It also interacts with chromatin components, including DNA and nucleolar proteins, to coordinate chromosome condensation and segregation during metaphase and anaphase.

The NUSAP1 gene, located on chromosome 15q15.1, encodes a cell cycle-regulated protein that peaks in expression during G2/M phase and is degraded after mitosis. NUSAP1 is phosphorylated by CDK1, controlling its localization and activity. It functions in concert with kinesin and dynein motors, contributing to spindle microtubule dynamics and mitotic checkpoint signaling.

Pathologically, elevated NUSAP1 expression is a hallmark of proliferating cells and various cancers, including breast, prostate, and lung carcinoma. Its overexpression correlates with increased tumor aggressiveness and poor prognosis, reflecting enhanced proliferative capacity. Conversely, NUSAP1 depletion leads to spindle defects, mitotic arrest, and genomic instability. Research with NUSAP1 antibody provides insight into cell division mechanisms and cancer cell cycle regulation.

NUSAP1 antibody is suitable for use in western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindles and cell cycle progression. NSJ Bioreagents supplies validated NUSAP1 antibody reagents ideal for studies of mitosis, chromosomal segregation, and cancer cell biology.

Structurally, NUSAP1 contains a coiled-coil domain, a microtubule-binding motif, and a DNA-binding region, which together mediate its dual role in chromatin attachment and microtubule bundling. This antibody supports exploration of NUSAP1�s role as a spindle scaffold that integrates chromosomal and cytoskeletal control during mitosis.