NUDT9 Antibody / ADP-ribose pyrophosphatase, 100 ug

NUDT9 antibody detects ADP-ribose pyrophosphatase, a member of the nudix hydrolase family that hydrolyzes ADP-ribose into AMP and ribose-5-phosphate, maintaining intracellular nucleotide balance. Encoded by the NUDT9 gene on chromosome 4q22.1, this enzyme regulates ADP-ribose metabolism and contributes to NAD+ turnover, calcium signaling, and oxidative stress responses. NUDT9 ensures proper degradation of ADP-ribose derived from poly(ADP-ribose) polymerase (PARP) activity following DNA damage.

Structurally, NUDT9 is a 350-amino-acid mitochondrial enzyme of approximately 40 kilodaltons containing a conserved Nudix box motif (GX5EXEEAXEE) that coordinates magnesium ions for catalytic hydrolysis of ADP-ribose. The enzyme localizes primarily to mitochondria and cytoplasm, where it prevents accumulation of free ADP-ribose that could perturb energy metabolism and calcium homeostasis. Its enzymatic activity helps recycle components of NAD+ metabolism and mitigate oxidative stress.

The NUDT9 antibody is widely used in biochemistry, metabolism, and mitochondrial biology research to study nucleotide turnover, ADP-ribose signaling, and cellular energy regulation. Western blot analysis detects a 40 kilodalton band corresponding to NUDT9, while immunofluorescence reveals mitochondrial and cytosolic localization. This antibody supports studies exploring enzymatic regulation of NAD+ pathways and mitochondrial nucleotide dynamics.

Functionally, NUDT9 plays a protective role against oxidative stress by regulating levels of ADP-ribose, a molecule that can activate TRPM2 channels and trigger calcium influx during stress responses. Dysregulation of NUDT9 expression affects calcium signaling, redox balance, and cell viability. Homologous enzymes and domains, such as those found in the TRPM2 channel, share structural similarity with NUDT9 and extend its functional relevance to ion channel regulation. The NUDT9 antibody provides a sensitive reagent for investigating nucleotide metabolism, mitochondrial stress adaptation, and PARP-related signaling. NSJ Bioreagents validates this antibody for its applications, ensuring accurate detection for cellular metabolism research.