POLD1 Antibody / DNA polymerase delta catalytic subunit, 100 ug

POLD1 antibody detects DNA polymerase delta catalytic subunit, the core enzyme responsible for high-fidelity DNA synthesis and repair during replication. Encoded by the POLD1 gene on chromosome 19q13.33, this enzyme serves as the catalytic and proofreading component of DNA polymerase delta, a multi-subunit complex essential for lagging-strand synthesis, mismatch repair, and genomic stability. POLD1 possesses both 5'-3' polymerase and 3'-5' exonuclease proofreading activities, ensuring accurate DNA replication and error correction.

Structurally, POLD1 is a large enzyme of approximately 125 kilodaltons containing conserved polymerase motifs and an N-terminal exonuclease domain. It forms a heterotetrameric complex with POLD2, POLD3, and POLD4 subunits, supported by interactions with PCNA (proliferating cell nuclear antigen) and replication factor C. This complex coordinates DNA elongation, repair synthesis, and recombination processes, playing a vital role in cell cycle progression and genome maintenance.

The POLD1 antibody is widely used in molecular biology, cancer research, and DNA repair studies to investigate replication fidelity, mutagenesis, and polymerase function. Western blot analysis detects a 125 kilodalton band corresponding to the catalytic subunit, while immunofluorescence shows nuclear localization during S phase and DNA damage repair. This antibody provides a robust tool for assessing DNA replication machinery and the maintenance of genomic integrity.

Mutations in POLD1 cause Polymerase Proofreading-Associated Polyposis and predispose to colorectal and endometrial cancers due to defective proofreading activity and increased mutational load. Additionally, POLD1 variants are linked to syndromes involving premature aging and mitochondrial dysfunction. Its role extends beyond replication to DNA damage tolerance and translesion synthesis. The POLD1 antibody supports exploration of polymerase fidelity mechanisms, DNA repair pathways, and tumor suppressor networks involving replication stress. NSJ Bioreagents validates this antibody for its applications, providing reliability and specificity for genomic stability research.