OTUD4 Antibody / OTU domain-containing protein 4, 100 ug

OTUD4 antibody detects OTU domain-containing protein 4, a deubiquitinating enzyme that regulates protein stability, DNA damage response, and innate immunity. Encoded by the OTUD4 gene on chromosome 4q31.23, this enzyme belongs to the ovarian tumor (OTU) family of cysteine proteases that cleave ubiquitin chains from substrate proteins. OTUD4 maintains cellular protein homeostasis by removing polyubiquitin modifications that target proteins for proteasomal degradation.

Structurally, OTUD4 contains an OTU catalytic domain with conserved cysteine and histidine residues required for thiol protease activity. It also possesses multiple ubiquitin-binding motifs that enable recognition of specific ubiquitin linkages, conferring substrate selectivity. OTUD4 localizes to both cytoplasm and nucleus, where it modulates key signaling proteins involved in stress response, interferon signaling, and genome maintenance.

The OTUD4 antibody is widely used in ubiquitin signaling, oncology, and immunology research to study protein degradation, stability, and DNA repair. Western blot analysis identifies an approximately 130 kilodalton band corresponding to OTUD4, while immunofluorescence reveals both cytoplasmic and nuclear punctate localization, consistent with its regulatory role in ubiquitin metabolism. This antibody enables detection of dynamic changes in ubiquitin-modifying enzymes during stress and immune signaling.

Functionally, OTUD4 stabilizes DNA repair proteins such as KU80 and RPA1, promoting efficient double-strand break repair. It also regulates antiviral responses by modulating the ubiquitination of MAVS and other interferon signaling components, thereby balancing antiviral defense and immune homeostasis. Loss or mutation of OTUD4 impairs genome integrity and contributes to increased sensitivity to genotoxic stress. In cancer, dysregulated OTUD4 expression affects tumor cell proliferation and chemoresistance by altering protein turnover and checkpoint signaling. The OTUD4 antibody provides an essential tool for exploring deubiquitination pathways and their role in cell signaling and genomic maintenance. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring accurate and reproducible detection for ubiquitin and DNA repair studies.