FUNDC1 Antibody / FUN14 domain-containing protein 1, 100 ug

FUNDC1 antibody detects FUN14 domain-containing protein 1, a mitochondrial outer membrane protein that regulates mitophagy and mitochondrial quality control. Encoded by the FUNDC1 gene on chromosome Xq26.1, this protein serves as a receptor for hypoxia-induced mitophagy, linking damaged or depolarized mitochondria to the autophagic machinery. FUNDC1 interacts with LC3 through its LC3-interacting region (LIR) motif, facilitating selective removal of dysfunctional mitochondria during cellular stress.

Structurally, FUNDC1 contains three transmembrane helices that anchor it to the mitochondrial outer membrane, with its N-terminal domain exposed to the cytosol for binding autophagy-related proteins. Under normoxic conditions, FUNDC1 is phosphorylated at Tyr18 and Ser13, suppressing its interaction with LC3. During hypoxia or mitochondrial depolarization, phosphatases such as PGAM5 dephosphorylate these residues, enhancing its affinity for LC3 and triggering mitophagy. This dynamic post-translational regulation ensures precise control of mitochondrial turnover.

The FUNDC1 antibody is widely used in mitochondrial biology, autophagy, and metabolic research to study mitophagy signaling, organelle homeostasis, and cellular stress adaptation. Western blot analysis detects a 16 kilodalton band corresponding to FUNDC1, while immunofluorescence shows punctate staining along mitochondrial networks. This antibody enables researchers to monitor mitochondrial dynamics and degradation under conditions such as hypoxia, oxidative stress, or metabolic reprogramming.

FUNDC1-mediated mitophagy is essential for maintaining energy balance and preventing accumulation of damaged mitochondria that could release pro-apoptotic or inflammatory signals. Dysregulation of FUNDC1 contributes to diseases such as cardiac ischemia, neurodegeneration, and cancer, where impaired mitophagy affects cell survival and metabolic fitness. The FUNDC1 antibody provides a sensitive reagent for assessing mitophagic flux and mitochondrial remodeling. NSJ Bioreagents validates this antibody for its applications, ensuring reliable detection for studies on mitochondrial quality control and autophagic signaling.