NELFA Antibody / Negative elongation factor A, 100 ug

NELFA antibody detects Negative elongation factor A, a transcriptional regulator that controls RNA polymerase II pausing and transcription elongation. Encoded by the NELFA gene on chromosome 9q34.11, this protein is a core component of the negative elongation factor (NELF) complex, which also includes NELFB, NELFC/D, and NELFE subunits. Together, these proteins transiently inhibit RNA polymerase II progression downstream of transcription start sites, enabling proper promoter-proximal pausing, RNA capping, and regulated gene expression.

NELFA interacts with DSIF (DRB sensitivity-inducing factor) and phosphorylated RNA polymerase II to modulate transcriptional timing and elongation efficiency. Release from pausing is mediated by P-TEFb-dependent phosphorylation events that allow productive elongation. Through these mechanisms, NELFA coordinates transcriptional responses to developmental, environmental, and signaling cues.

The NELFA antibody is widely used in transcription, chromatin, and epigenetic regulation research to study RNA polymerase II pausing, elongation control, and transcriptional activation. Western blot analysis identifies a 66 kilodalton band corresponding to NELFA, while immunofluorescence reveals nuclear punctate staining associated with active transcription foci. This antibody supports studies investigating transcriptional regulation and pause-release mechanisms.

Altered NELFA activity affects global gene expression programs, contributing to developmental disorders, cancer, and viral replication control. NELFA has also been linked to stress responses, where it modulates transcription restart following DNA damage or heat shock. The NELFA antibody provides a critical reagent for dissecting the molecular mechanisms of transcription elongation and RNA polymerase II regulation. NSJ Bioreagents offers this antibody validated for its applications, ensuring dependable performance in transcriptional research.