CD300A Antibody, 100 ug

CD300A antibody detects Cluster of differentiation 300A, an inhibitory immunoreceptor expressed on myeloid and lymphoid cells that modulates immune activation and inflammation. Encoded by the CD300A gene on chromosome 17q25.1, this transmembrane glycoprotein belongs to the CD300 family of immunoglobulin-like receptors and plays a key role in maintaining immune homeostasis. CD300A contains a single extracellular Ig-like domain that recognizes phosphatidylserine and other lipids exposed on apoptotic cells, allowing immune cells to distinguish between healthy and dying cells.

Structurally, CD300A possesses multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic tail. Upon ligand engagement, these motifs recruit phosphatases such as SHP-1 and SHP-2, which dephosphorylate signaling intermediates and suppress proinflammatory responses. This mechanism dampens activation of monocytes, macrophages, mast cells, and NK cells, preventing excessive inflammation and autoimmune reactions. CD300A thereby acts as a negative regulator of immune activation by balancing activating signals from other CD300 family members.

The CD300A antibody is widely used in immunology, inflammation, and autoimmune disease research to study inhibitory receptor signaling, immune regulation, and apoptotic cell clearance. Flow cytometry and western blot analyses detect a 35-40 kilodalton band corresponding to CD300A, while immunohistochemistry shows strong membrane staining on leukocytes and myeloid cells. This antibody supports analysis of inhibitory receptor expression and function in both innate and adaptive immune systems.

Aberrant CD300A expression has been implicated in autoimmune diseases, allergy, and cancer. Increased expression can suppress antitumor immunity, while reduced expression contributes to hyperinflammatory conditions. The CD300A antibody provides a valuable tool for investigating the balance between immune activation and inhibition in health and disease. NSJ Bioreagents validates this antibody for its applications, ensuring reproducibility and sensitivity in immune signaling research.