ORC2 Antibody / Origin recognition complex subunit 2, 100 ug

ORC2 antibody detects Origin recognition complex subunit 2, a component of the six-subunit origin recognition complex (ORC) that initiates DNA replication. ORC2 binds replication origins throughout the genome, marking them for pre-replicative complex assembly and ensuring precise duplication of the genome each cell cycle. The ORC2 antibody is used extensively in molecular biology, replication, and cell cycle research to study DNA replication initiation and checkpoint control.

ORC2 is encoded by the ORC2 gene on human chromosome 2q23.1. The protein is approximately 577 amino acids long and functions as part of the core ORC complex with ORC1, ORC3, ORC4, ORC5, and ORC6. ORC2 contributes to the stability and chromatin association of the complex, anchoring it to replication origins during G1 phase. Its binding provides a platform for recruitment of CDC6, CDT1, and the MCM helicase complex required for origin licensing.

The ORC2 antibody detects a 72 kilodalton protein by western blot and demonstrates nuclear punctate staining consistent with replication foci. ORC2 remains bound to chromatin through most of the cell cycle, although its activity is regulated by phosphorylation and proteasomal turnover. Disruption of ORC2 function leads to replication defects, genomic instability, and cell cycle arrest.

In addition to replication initiation, ORC2 participates in heterochromatin organization and transcriptional silencing. ORC2 interacts with chromatin modifiers such as HP1 and histone methyltransferases to maintain heterochromatin boundaries. Deficiency of ORC2 causes chromosomal breaks and increased sensitivity to replication stress, linking replication initiation to genome integrity.

Because ORC2 is fundamental to DNA replication and chromatin architecture, it serves as a valuable marker for replication initiation studies. NSJ Bioreagents provides a validated ORC2 antibody optimized for western blot, immunofluorescence, and immunohistochemistry, supporting research into DNA replication origin function, licensing, and genomic maintenance.