RNF111 Antibody / RING finger protein 111, 100 ug

RNF111 antibody detects RING finger protein 111, an E3 ubiquitin-protein ligase that regulates DNA damage signaling, TGF-beta signaling, and transcriptional control. Also known as Arkadia, RNF111 facilitates ubiquitin-dependent degradation of transcriptional repressors to enhance signal transduction. The RNF111 antibody is used in studies of ubiquitin signaling, DNA repair, and cancer biology.

RNF111 is encoded by the RNF111 gene on human chromosome 15q22.33. The protein is approximately 101 kilodaltons and contains a RING finger domain, two nuclear localization signals, and several coiled-coil regions. It acts as an E3 ligase that mediates ubiquitination of SMAD7 and SKI, thereby amplifying TGF-beta and BMP signaling cascades that control differentiation and tissue remodeling.

The RNF111 antibody identifies a 100-105 kilodalton nuclear protein by western blot. Immunofluorescence reveals punctate nuclear distribution consistent with its role in transcriptional regulation. RNF111 participates in DNA damage response through ubiquitination of SUMO-modified proteins at sites of double-strand breaks, promoting recruitment of repair factors. Loss or mutation of RNF111 results in defective DNA repair and impaired transcriptional activation of TGF-beta-responsive genes.

RNF111 also modulates embryonic development, neural differentiation, and tumor suppression. Overexpression has been associated with hepatocellular carcinoma, whereas downregulation disrupts differentiation signaling in stem cells. NSJ Bioreagents provides a validated RNF111 antibody, supporting investigation of ubiquitin signaling, DNA repair pathways, and transcriptional regulation.