POLD4 Antibody / DNA polymerase delta subunit 4, 100 ug

POLD4 antibody detects DNA polymerase delta subunit 4, encoded by the POLD4 gene on chromosome 11q13.4. POLD4 antibody is widely applied in DNA replication, repair, and cell cycle research. POLD4 is a small accessory subunit of DNA polymerase delta, a multi-subunit complex essential for lagging strand DNA synthesis, Okazaki fragment maturation, and DNA repair. POLD4 stabilizes the polymerase complex and enhances its processivity in conjunction with PCNA and replication factor C.

Structurally, POLD4 is a ~12 kDa protein with no catalytic activity but essential stabilizing functions. It interacts with the catalytic subunit POLD1 and accessory subunits POLD2 and POLD3, forming the tetrameric polymerase delta complex. By supporting proper assembly, POLD4 ensures high-fidelity DNA replication and repair.

Functionally, POLD4 contributes to DNA replication, mismatch repair, and recombination. It coordinates lagging strand synthesis, working with FEN1 and DNA ligase I to process Okazaki fragments. Researchers use POLD4 antibody to study DNA replication, checkpoint control, and repair pathways.

Clinically, defects in DNA polymerase delta subunits including POLD4 have been linked to cancer, immunodeficiency, and genomic instability syndromes. Reduced POLD4 expression impairs DNA replication fidelity, contributing to mutagenesis. Because DNA replication is a key therapeutic target, POLD4 is being investigated in oncology. NSJ Bioreagents provides POLD4 antibody for DNA replication, repair, and cancer studies.

Experimentally, POLD4 antibody is applied in western blotting to detect the ~12 kDa protein, in immunoprecipitation to isolate the polymerase delta complex, and in immunofluorescence to study nuclear localization. Chromatin immunoprecipitation with POLD4 antibody supports research on replication fork progression.